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Cytiva Europe column
Column, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hiprep+16+60+sephacryl+s+300+column/HiPrep+16%2F60+Sephacryl+S-300+HR+column/pmc13623949-477-35-36
Average 94 stars, based on 80 article reviews

column - by Bioz Stars, 2026-10

94/100 stars

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Purification:

Article Title: Physical basis for the loading of a bacterial replicative helicase onto DNA
Article Snippet: Wild-type DnaB was purified by ammonium sulfate precipitation (30% wt/v), followed by resuspension of the pellet in 100 mM NaCl Q Buffer(100 mM NaCl, 20 mM Hepes pH 7.5, 10 % glycerol, 10 mM MgCl 2, 0.01 mM ATP, 1 mM BME, and protease inhibitors) and passage over an HiTrap Q HP anion exchange column (GE Healthcare). .. Peak fractions were pooled, concentrated in Amicon Ultra Centrifugal Filters 30,000 MWCO (Millipore) and further purified by gel filtration on a HiPrep 16/60 Sephacryl S-300 column (GE Healthcare) in buffer containing 20 mM Tris-HCl (pH 8.5), 800 mM NaCl, 10% glycerol, 5 mM MgCl2, 1 mM β-mercaptoethanol, 0.1 mM ATP, 1 mM PMSF, 1 μg/mL Pepstatin A, and 1 μg/mL Leupeptin. ..

Article Title: The molecular coupling between substrate recognition and ATP turnover in a AAA+ hexameric helicase loader
Article Snippet: Clarified lysates containing wild-type DnaB were ammonium sulfate precipitated (30% wt/v), followed by resuspension of the pellet in 100 mM NaCl Q Buffer (100 mM NaCl, 20 mM HEPES-KOH (pH 7.5), 10% glycerol, 10 mM MgCl2, 0.01 mM ATP, 1 mM b-mercaptoethanol, and protease inhibitors), and passed over a HiTrap Q HP anion exchange column (GE Healthcare). .. Peak fractions were pooled, concentrated in Amicon Ultra Centrifugal Filters (30,000 MWCO; Millipore), and further purified by gel filtration on a HiPrep 16/60 Sephacryl S-300 column (GE Healthcare) in a buffer containing 20 mM Tris-HCl (pH 8.5), 800 mM NaCl, 10% glycerol, 5 mM MgCl2, 1 mM bmercaptoethanol, 0.1 mM ATP, 1 mM PMSF, 1 mg/ml pepstatin A, and 1 mg/ml leupeptin. ..

Article Title: The molecular coupling between substrate recognition and ATP turnover in a AAA+ hexameric helicase loader
Article Snippet: Clarified lysates containing wild-type DnaB were ammonium sulfate precipitated (30% wt/v), followed by resuspension of the pellet in 100 mM NaCl Q Buffer (100 mM NaCl, 20 mM HEPES-KOH (pH 7.5), 10 % glycerol, 10 mM MgCl 2, 0.01 mM ATP, 1 mM β-mercaptoethanol, and protease inhibitors) and passed over an HiTrap Q HP anion exchange column (GE Healthcare). .. Peak fractions were pooled, concentrated in Amicon Ultra Centrifugal Filters (30,000 MWCO, Millipore) and further purified by gel filtration on a HiPrep 16/60 Sephacryl S-300 column (GE Healthcare) in buffer containing 20 mM Tris-HCl (pH 8.5), 800 mM NaCl, 10% glycerol, 5 mM MgCl 2 , 1 mM β-mercaptoethanol, 0.1 mM ATP, 1 mM PMSF, 1 μg/mL Pepstatin A, and 1 μg/mL Leupeptin. ..

Article Title: Methods of culturing and characterizing antibody secreting cells
Article Snippet: Biotinylated proteins were then tetramerized with fluorochrome-labeled streptavidin (Prozyme, Hayward, Calif.). .. Labeled tetramers were purified by size exclusion on a HiPrep 16/60 Sephacryl S-300 column (GE Healthcare, Piscataway, N.J.). ..

Article Title: A transcriptionally distinct subset of influenza-specific effector memory B cells predicts long-lived antibody responses to vaccination in humans.
Article Snippet: In brief Seasonal influenza vaccination recalls pre-existing memory B cells for immune protection.. Here, Nellore et al. show that intramuscular influenza vaccination elicits a population of effector memory B cells that are transcriptionally, epigenetically, and metabolically poised for antibody production.. The magnitude of this FcRL5and T-bet-expressing effector memory subset response correlates with long-lived antibody responses to influenza vaccination.

Filtration:

Article Title: The molecular coupling between substrate recognition and ATP turnover in a AAA+ hexameric helicase loader
Article Snippet: Clarified lysates containing wild-type DnaB were ammonium sulfate precipitated (30% wt/v), followed by resuspension of the pellet in 100 mM NaCl Q Buffer (100 mM NaCl, 20 mM HEPES-KOH (pH 7.5), 10 % glycerol, 10 mM MgCl 2, 0.01 mM ATP, 1 mM β-mercaptoethanol, and protease inhibitors) and passed over an HiTrap Q HP anion exchange column (GE Healthcare). .. Peak fractions were pooled, concentrated in Amicon Ultra Centrifugal Filters (30,000 MWCO, Millipore) and further purified by gel filtration on a HiPrep 16/60 Sephacryl S-300 column (GE Healthcare) in buffer containing 20 mM Tris-HCl (pH 8.5), 800 mM NaCl, 10% glycerol, 5 mM MgCl 2 , 1 mM β-mercaptoethanol, 0.1 mM ATP, 1 mM PMSF, 1 μg/mL Pepstatin A, and 1 μg/mL Leupeptin. ..

Labeling:

other:

Article Title: Engineered cascade components and cascade complexes
Article Snippet: The concentrated sample was filtered using a 0.22 μM Ultrafree-MC GV Centrifugal Filter (Millipore Sigma, Billerica, Mass.) before being further purified by separation at 4° C. with a flow rate of 0.5 mL/minute on a HiPrepTM 16/60 Sephacryl® S-300 column (GE Healthcare, Uppsala, Sweden) equilibrated with SEC buffer composed of 50 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 0.1 mM EDTA, and 1 mM TCEP.

Article Title: Engineered cascade components and cascade complexes
Article Snippet: The concentrated sample was filtered using a 0.22 μm 13 mm UltraCruz® PVDF syringe filter (Santa Cruz Biotechnology, Dallas, Tex.) before being further purified by separation at 4° C. with a flow rate of 0.4 mL/minute on a HiPrepTM 16/60 Sephacryl® S-300 column (GE Healthcare, Uppsala, Sweden) equilibrated with SEC buffer composed of 50 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 0.1 mM EDTA, and 1 mM TCEP.

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